CV Célia Leão

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  Célia Leão

  Investigadora de Pós-Doutoramento                

  Doutorada em Biologia

  FCT, Universidade Nova de Lisboa

 

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  Tel: +351 284314399

  Ext: 01107/01105 

 

 

Education and Training   

Ph.D degree in Biology – specialization in Microbiology, Faculdade de Ciências e Tecnologia - Universidade Nova de Lisboa, Caparica, Portugal, 2015

Bachelor's degree in Microbiology, Escola Superior de Biotecnologia - Universidade Católica Portuguesa, Caldas da Rainha, Portugal, 2008

Research and Professional Experience

Feb. 2017 – Present Post-Doctoral Researcher

CEBAL -Centro de Biotecnologia Agrícola e Agro-alimentar do Alentejo, Beja, Portugal. Project ALT20-03-0145-FEDER-000041 SelectPinea – Development of genetic markers methodology for selected traits of interest in Pinus pinea.

Oct. 2010 – Dec. 2015 PhD student

INIAV, IP - Instituto Nacional de Investigação Agrária e Veterinária, I.P., Departamento de Saúde Animal, Lisboa, Portugal. SFRH/BD/62469/2009 - Molecular tools in the diagnostic and epidemiology of infections caused by members of Mycobacterium avium Complex.

Current interests of research

Bioinformatics, Next-Generation-Sequencing, Molecular Biology and Genetics, Genotyping, Infectious Diseases, Microbiology

Selected Publications

Papers in peer reviewed journals

Leão C, Goldstone RJ, Bryant J, McLuckie J, Inácio J, Smith DGE, Stevenson K. 2016. Novel SNP-based assay for genotyping Mycobacterium avium subsp. paratuberculosis. J Clin Microbiol 54:556-564 (doi: 10.1128/JCM.01958-15).

Leão C, Amaro A, Santos-Sanches I, Inácio J, Botelho A. 2015. Paratuberculosis asymptomatic cattle as spillovers of Mycobacterium avium subsp. paratuberculosis: consequences for disease control. Rev Port Ciênc Vet 110: 69-73.

Leão C, Canto A, Machado D, Santos-Sanches I, Couto I, Viveiros M, Inácio J, Botelho A. 2014. Relatedness of Mycobacterium avium subspecies hominissuis clinical isolates of human and porcine origins assessed by MLVA. Vet Microbiol 173:92-100 (doi:10.1016/j.vetmic.2014.06.027).

Gomes J., Leão C., Ferreira F., Afonso M. O., Santos C., Josenando T., Seixas J., Atouguia J., Centeno-Lima S. 2009. Molecular identification of T. brucei s.l. in tsetse flies after long-term capture in field traps. Journal of Infection Developing Countries 3(9): 735-738.

Book Chapter

Botelho A, Canto A, Leão C, Cunha MV. 2014 Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) analysis of members of the Mycobacterium tuberculosis complex. In: Cunha MV, Inácio J (Eds) Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies. Springer Science + Business Media, LLC, NY, USA [Chapter in an international book: chapter 27] 1247:373-389 (doi: 10.1007/978-1-4939-2004-4_27).

Oral Presentations

Celia Leão, Robert J Goldstone, Josephine Bryant, Joyce McLuckie, David GE Smith and Karen Stevenson (2014, June) Novel SNP-based analysis for the differentiation of isolates of Mycobacterium avium subsp. paratuberculosis. Oral communication at 12th International Colloquium on Paratuberculosis Parma, Italy.

Soares R., Santos M., Varela F., Costa P., Ferreira A., Leão C., Gomes J., Botelho A., Amaro A., Baptista P., Prazeres M., Fortunato E. and Inácio J.. 2011. Emerging instrument-free diagnostic tools for the molecular detection of zoonotic pathogens. Oral communication (by Inácio J.), II Jornadas de Saúde Pública Veterinária, 28th May, Vila Real, Portugal.

Poster in Conferences

Célia Leão, Ana Botelho, Elisabete Martins, Carla Aguiar, Telmo Nunes, Inês Rebelo, Ricardo Bexiga (2016, July) Presence of Mycobacterium avium subs. paratuberculosis DNA in milk used to feed calves in Portugal. Poster presented at World Buiatrics Congress 2016, Dublin, Ireland.

C. Leão, P. Barbosa, A. Usié, I. Chaves, A. Amaro, A. Botelho, C. Pinto, J. Inácio, K. Stevenson, A.M. Ramos (2016, June) Molecular characterization of a rare pigmented Mycobacterium avium subsp. paratuberculosis Type C strain. Poster presented at 13th International Colloquium on Paratuberculosis, Nantes, France.

P. Barbosa, C. Leão, A. Usié, I. Chaves, A. Amaro, A. Botelho, C. Pinto, K.Stevenson, J. Inácio, A.M. Ramos (2016, February) Genome wide comparison between Mycobacterium avium subsp. paratuberculosis non-pigmented and pigmented type C isolates. Poster presented at 5th Bioinformatics Open Days 2016, Universidade do Minho, Braga, Portugal.

Ana Reis, Célia Leão, Victor Bandeira, Carlos Fonseca, Mónica V. Cunha (2015, June) On the trail of Mycobacterium avium subsp. paratuberculosis. Poster presented at Frontiers in E3, cE3c Annual Meeting 2015, Faculdade de Ciências, Lisbon University, Portugal.

Amaro A., Nunes M., Gomes J., Leão C., Inácio J. and Vieira M.L (2015, April) Molecular investigation of Borrelia burgdorferi sensu lato in serum of several animal species from mainland Portugal. Poster presented at EurNegVec, Izmir, Turkey.

Célia Leão, Ana Amaro, Carlos Pinto, Ana Botelho e João Inácio (2014, June) Semi-nested probe-based real-time PCR for the detection of Mycobacterium avium subsp paratuberculosis in goats. Poster presented at 12th International Colloquium on Paratuberculosis Parma, Italy.

Leão C., Amaro A., Botelho A., Inácio J. (2014, April) Screening of asymptomatic Portuguese cattle for the presence of Mycobacterium avium subsp. paratuberculosis in fecal samples. Poster presented at VI Congresso da Sociedade Portuguesa de Ciências Veterinárias - CCV2014, Oeiras, Portugal.

Leão C, Amaro A, Santos Sanches I, Botelho A, Inácio J. (2013, December) Assessment of Mycobacterium avium subs. paratuberculosis faecal shedding in asymptomatic Portuguese cattle. Poster presented at Portuguese Congress of Microbiology and Biotechnology' 2013, Aveiro, Portugal.

Leão C, Amaro A, Santos Sanches I, Botelho A, Inácio J. (2011, June) Assessment of Mycobacterium avium subsp. paratuberculosis infection in asymptomatic Portuguese cattle using molecular and culture-based assays. Poster presented at XVII World Buiatrics Congress 2012, Lisbon, Portugal.

Célia Leão, João Inácio, Ilda Sanches and Ana Botelho. (2011, December) Genetic heterogeneity of Mycobacterium avium subspecies hominissuis isolated in Portugal. Poster presented at Microbiotec 2011, Portuguese Society of Microbiology and Biotechnology, Braga, Portugal.

Célia Leão, João Inácio, Ilda Sanches and Ana Botelho. (2011, June) Evaluation of Mycobacterium avium tandem repeat (MATR) typing of M. avium subsp. hominissuis strains with a high diversity of IS1245-RFLP profiles. Poster presented at ESM 2011, Lübeck, Alemanha.

Thesis

Célia Leão. 2015. Molecular tools in the diagnostic and epidemiology of infections caused by members of Mycobacterium avium Complex. PhD thesis. Faculdade de Ciências e Tecnologia da Universidade Nova de lisboa, Lisbon, Portugal. (http://hdl.handle.net/10362/16513)

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